Rotavirus, is the leading cause of diarrhoeal disease mortality
among children under five, resulting in 450,000 to 700,000 deaths
each year, and another 2 million hospitalisations, mostly in the
developing world. By the age of five, nearly every child in the
world has been infected with rotavirus at least once. It is
transmitted by the faecal-oral route, via contact with contaminated
hands, surfaces and objects. It is very stable in the environment
and resistant to most household/clinical biocides.
The faeces of an infected person can contain more than 10 trillion
infectious particles per gram and only 10–100 of these are
required to transmit infection to another person. With no antiviral
treatment available, management of the child must focus on
prevention of dehydration.
Inhibition of Rotavirus replication can be monitored by measuring
cell viability by the MTT assay using mammalian cell culture.
If you are interested in Retroscreen's antiviral assays for this
virus please click
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References:
Velázquez FR, Matson DO,
Calva JJ, Guerrero L, Morrow AL, Carter-Campbell S, Glass RI, Estes
MK, Pickering LK, Ruiz-Palacios GM (1996). "Rotavirus infections in
infants as protection against subsequent infections". N. Engl. J.
Med. 335 (14): 1022–8.
Butz AM, Fosarelli P, Dick J, Cusack T, Yolken R (1993). "Prevalence of rotavirus on high-risk fomites in day-care facilities". Pediatrics 92 (2): 202–5.
Graham DY, Dufour GR, Estes MK (1987). "Minimal infective dose of rotavirus". Arch. Virol. 92 (3–4): 261–71.
O Nakagomi, NA Cunliffe (2007). “Rotavirus vaccines:
entering a new stage of deployment”. Curr Opin Infect Dis. 20
(5): 501-7.